Tom,
Turn off the Optimize option in the Master Dark pane of ImageCalibration and rerun. See the pic below.
With a single frame as dark master, PI dark optimization does not do a good job on your frames. It finds a scaling factor of about 0.2 rather than a value near 1.0, as it should be for equal exposure light and dark and no bias specified.
With the Optimized turned off, ImageCalibration will simply do the calculation light - dark and give nearly the same results as the PixelMath sanity check I posted previously.
Note: even when using a master dark, you may find turning off Optimize works better when dark/light exposure times match. My NB lights and dark masters are 2400s and I find no optimize works better. I think Juan is working on improving dark optimization so things should be better eventually.
Mike