actually, if you have an Ha image from an OSC for which you are only interested in the red channel, you can debayer first as long as you use SuperPixel mode, and extract the red channel as a mono file. then you are still dealing with the raw red pixel values, and if all your subs were preprocessed this way then you can make masters and calibrate the lights.
if you don't use superpixel debayering, then this is not correct as the interpolation of the missing R pixels will be done before calibration. but with superpixel, there is no interpolation. the red pixel at 0,0 goes to the output image at 0,0, the red pixel at 2,0 goes to the output image at 1,0 and so on. this is why superpixel-debayered OSC images are 1/2 the pixel dimensions of the sensor.
anyway Gerald is absolutely right. you need good signal strength in each channel of your flat (or those channels that you care about.) if the SNR of the flats is low, then this low SNR corrupts your calibrated frames.
i am not sure if PI calculates a scaling factor for each flat channel individually. certainly for CFA images this is hard to do, but not impossible. if it does, then low SNR is your only problem. if it does not, then since the signal is very low in the R channel the scaling will probably be wrong and one or more channels will be under or over corrected.
rob