I've found PI's calibration routines fairly easy to use, and the tutorials on Darks, Biases, and Flats very helpful. Perhaps this will help.
I assume you are doing LRGB imaging and your luminance is binned 1x1 and the RGB is binned 2x2.
Your raw imaging frames have artifacts from the imaging train (vignetting, dust, etc.), baseline noise from the CCD, and dark noise from heat and imaging time. You need to remove these from your images.
Flats correct artifacts from the imaging train, and darks and biases, the other two noise factors.
I assume you've taken flats, darks, and bias frames in addition to your lights.
Combine -- Image Integration -- the darks and bias per the instructions in the tutorial. These are the master darks and biases. Then calibrate -- Image Calibration -- the flats images. Since a good flat is at about 50% average full ADU depth on your CCD, if you aren't taking sky flats, 4 or so flats should be adequate. If you aren't taking sky flats, calibrate the flats using your master dark and bias and do a simple mean. Now you have master darks, bias, and flats.
Use image calibration to calibrate your frames, using appropriate flats (one for each filter and rotation), darks and bias frames. I count on PI to adjust darks for temperature and time.
Then align your luminance frames with StarAlignment. Use Image integration to combine the aligned luminance frames. Use appropriate pixel rejection to eliminate outliers. The help on this is very good. Save your integration, then use IntegerResample to downscale the luminance to a 2x2 size to serve as the master alignment frame for the RGB.
Align the RGB to the binned luminance image, and I would recommend setting the interpolation on the RGB to nearest neighbor, assuming you have at least 6 color frames. You should also be dithering your captured image to move any CCD artifacts around the actual image. Combine the frames using the best pixel rejection scheme.
At that point, you have the ingredients to follow the excellent video tutorial on LRGB combination.
I hope this helps.
Clear skies,
--Andy