Hi Emanuele,
OK - here is my 'thought experiment for the day'
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Ensure that your CCD is temperature stabilised
Shoot five darks at X seconds exposure time - however, from this point on you will treat these as 'Lights'
Shoot an appropriate number of Biases
Shoot an appropriate number (say ten, each) of Darks at X seconds, (2 x X) seconds, (3 x X) seconds (etc.) - you could even try with (0.5 x X) seconds as well
Now, use PI to calibrate the original set of 'Lights' - do this for each differing source of Darks. Then perform a simple ImageIntegration of the results (no pixel rejection, etc. - just 'Averaging')
In an 'ideal' world - when you calibrate your lights with the Darks taken at the same exposure, you should (in theory) end up with a master frame that is effectively TOTALLY DARK (or at least with all pixels having the same ADU value, where this value 'represents total dark' for your CCD)
When you calibrate your Lights with the other Darks, you 'should' end up with the same result. Providing, of course, that the Dark Optimisation routine was able to correctly scale your 'exposure-time-mismatched' Darks.
This is not a difficult experiment to carry out - and is ideally suited to 'down-time' when the weather is not playing fair.
Let us know how you get on (just remember that the 'Lights' accumulated 'in the dark' must obviously be totally different from any 'real Darks' acquired for the same exposure time - this is a requirement for a valid statistical analysis)
Cheers,