As I understand things Jack (and I am just about to sit down to my 'first run through'),
You start off with raw Lights, Darks, Flats and Bias frames
The first two steps are both ImageIntegration (II) steps, and these allow you to create a MasterDark and a MasterBias frames from your raw subs - you cannot, at this stage, create the MasterFlat because you will need BOTH the MasterDark and the MasterBias frame to pre-calibrate your Flats.
The next stage is to then use the ImageCalibration (IC) process, along with your new MasterDark and MasterBias frames, to calibrate the INDIVIDUAL Flat frames
Then you need to use II again, this time to integrate (stack) your newly calibrated Flat frames into a MasterFlat.
Only now do you have the three (master) calibration frames, which can now be applied to your Lights - again using the new IC process. as far as the tutorial then goes, you do NOT tick the 'calibrate' check box in the 'Master Flat' sub-section. The reason being that you have already just 'calibrated' the Flats.
The bit that I don't yet understand is why - at the time we are about to 'calibrate' the Lights - do we still then have the 'calibrate' box ticked for the 'Master Bias' and the 'Master Dark' sections
I need to go and 'have a play' - and see if I can work out the logic.
Cheers,