H Mirco,
Used camera: Canon EOS 550D (14-bit ADC, FITS -> RGGB).
Statistics of the superbias compared with a dark frame:
superbias
count (%) 100.00000
count (px) 18024930
mean 2048.359
median 2048.562
variance 1.738
stdDev 1.318
avgDev 1.406
MAD 1.641
minimum 2044.464
maximum 2052.339
Dark frame: ic434_2019_12_31_240sec_ISO800_281_amb_0_4C_dark_frame9
count (%) 100.00000
count (px) 18024930
mean 8198.4
median 8195.0
variance 20122.6
stdDev 141.9
avgDev 56.7
MAD 53.4
minimum 7239.0
maximum 61223.0
The median of the superbias is 2048.6, the median of the single dark frame is 8195. My conclusion: The superbias was generated from NOT scaled bias frames (presumably from bias frames in CR2 format). So the superbias does not match all the other frames.
I made the following steps with your frames (just quick and dirty -- not to be recommended):
Multiplied (in PixelMath) the superbias by factor 4.0, calibrated the flat frames with this modified superbias and integrated the calibrated flat frames to a MasterFlat. Integrated the dark frames to a MasterDark. Calibrated the light frames with the MasterDark (both 'Calibrate' and 'Optimize' disabled) and the MasterFlat ('Calibrate' disabled).
The image that resulted after debayering, star alignment and integration (see appended integration.JPG) has a bright stripe (of about 230 pixels in height) at the bottom. This artifact is in all calibrated lights as well, so it does not result from dithering or drift. It could be caused by the fact that the red channel in the flat frames is very dim. Apart from that, the integration result doesn't look wrong.
Suggestions:
1) Delete the superbias.
2) If your bias frames are in CR2 format (this is the only reasonable explanation for the above finding), delete them.
3a) If you don't want to try dark frame optimization, you need not capture bias frames at all. In this case, take flat darks (same ISO and exposure as the flat frames), integrate them to a MasterFlatDark and use this MasterFlatDark for flat frame calibration. Integrate the calibrated flat frames to the new MasterFlat.
Calibrate the light frames with the MasterDark (both 'Calibrate' and 'Optimize' disabled) and the MasterFlat ('Calibrate' disabled).
3b) If you want to try dark frame optimization, In this case, take new bias frames with SGP in FITS format, integrate them to a MasterBias and use this MasterBias for flat frame calibration. Integrate the calibrated flat frames to the new MasterFlat.
Calibrate the light frames with the MasterDark (both 'Calibrate' and 'Optimize' enabled) and the MasterFlat ('Calibrate' disabled).
Compare the resulting integrations, and for the future use the process that provides superior results (either 3a) or 3b)).
Bernd