Of course, Mike's conclusion about reference frames in ImageIntegration is correct: The choice of a particular reference frame has no repercussion on the SNR improvement achieved in the final result, other than insignificant differences caused by roundoff errors. As Mike describes, this is because II applies only linear transformations to the data—it could not be otherwise, or linearity of the integrated image would be damaged. These conclusions can be drawn from the reference documentation for II, which (although now outdated) includes detailed descriptions of the algorithms and procedures implemented in the II process.
Reference choice affects the location (median) and scale (the selected II scale method) of the result. So in a sense the result "looks" more like the chosen reference in these two aspects (ie a similar background level and a similar contrast).
This is true and is actually a 'feature' of the ImageIntegration process. This is in fact the main reason one may have to choose a particular integration reference frame in a given data set. Here we must stress the important fact that, within reasonable limits, location and scale statistics should have no practical influence on the proportions of signal and noise in the final result.
Note also that the PI's noise evaluation method (used by several scripts and II) also employs thresholding. I am worried that it might in some cases be overly sensitive to linear transforms and report a false reference choice difference.
All relevant internal calculations in the ImageIntegration process and native PJSR routines are performed in 64-bit floating point format, and care has been taken in the algorithm implementations to prevent accumulation of roundoff errors as possible. Within the limits of reasonable procedures, I doubt this may become a practical problem. Of course, if insensate decisions and selections are made (including, for example, the use of a hugely bad frame as reference), bad results are definitely possible.