Hi Mac:
Ok. I don't see the issue (being a source of light) in your dark... but I did notice a couple of things...
1. That dark has the extension of FIT. Is there anything else that was done to it? I almost looks like a single dark frame that yes, is normalized (0-1)- but something is weird. The dark should look like the master bias and have roughly the same kind of ADUs... (since the dark is a thermal signal plus the bias and those hot pixels). But your dark is an order of magnitude SMALLER in values compared to the bias. It appears as if the darks were bias subtracted and this is a thermal frame?? This would not be the standard way to process data- because PI (via BPP or perhaps your own hands on method) is expecting a dark that it will subtract a bias from. Looking at the FIT header I can see you combined 32 frames with the Linear Fit rejection method. Fine... but I think something happened before then? The difference in counts between your dark and flat needs to be explained. By the way, I am using the same chip.
2. Yes, I meant in MT...thanks.
3. A crease in a diffuser will not cause a feature like what you seeing. Your diffused sources of light are so out of focus... that black spots, holes...etc- really don't matter (with regards to this mystery anyway).
So, unfortunately I did not solve the mystery- but I wonder if can win a consolation prize if I am correct there is still something wrong with your darks.
Again, please let me know so I can exhaust all of my wrong guesses.
-adam