I have tried both method 1 and 2. 1 works fine with no hot pixels remaining. Here is the end of the log file with method 2:
2017-11-11_ngc151_Light_Lum_120sec_0001_-20C_c.xisf
Dark scaling factors:
k0 = 0.151
Gaussian noise estimates:
s0 = 8.925e-04, n0 = 0.697 (MRS)
Writing image: w=765 h=510 n=1 Gray Float32
53 FITS keyword(s) embedded.
This is method 1 log:
2017-11-11_ngc151_Light_Lum_120sec_0001_-20C_c.xisf
Gaussian noise estimates:
s0 = 8.847e-04, n0 = 0.738 (MRS)
Writing image: w=765 h=510 n=1 Gray Float32
49 FITS keyword(s) embedded.