I should pre-calibrate the master darks and not use a master bias in the light calibration.
No, you shouldn't, this could result in clipping of pixels in the precalibrated MasterDark. It is better NOT to precalibrate the MasterDark.
Try the process that I described as Case 1:
- no precalibration of the MasterDark,
- use MasterDark, no MasterBias,
- in the Master dark section of ImageCalibration uncheck both 'Calibrate' and 'Optimize' options.
Then try Case 2:
- no precalibration of the MasterDark,
- use MasterDark and MasterBias,
- in the Master dark section of ImageCalibration check both 'Calibrate' and 'Optimize' options.
Compare, which process makes the better SNR.
I notice if I use your recommended method 2 the standard deviation of the light is lower but I get many dark pixels throughout the image. Any idea what might cause this?
Was that really with an uncalibrated MaserDark and settings that I recommended for Case 2? That's odd. Usually there remain some hot pixels after a light frame calibration WITH dark frame optimization, and these then have to be eliminated with CosmeticCorrection.
Anyway - you definitely want to avoid dark pixels after calibration. If light frame calibration without dark frame optimization results in better calibrated light frames, it is recommendable to use this process. It might depend on the camera, whether dark frame optimization is favorable or not.
Bernd