Hi jerryyyyy,
The checkerboard pattern is an artifact due to excessive denoising. Typically one or more parameter is bad: Gain is too small; Gaussian noise is too big; Offset is nonzero (for calibrated images); Variance scale is too big.
To estimate Gaussian noise, pick two good bias frames (temperature matched to lights) and run DarkBiasNoiseEstimator. As a sanity check, you should get a noise estimate (in DN) roughly equal to manufacture specs "read noise" or "system noise" (in e-) divided by "gain" (in e-/DN). Or a little bit larger (say 10%) as detectors get a bit more noisy with age.
Longer light exposures will have additional noise due to dark current and preflash leakage (if any). So rather than using two biases for a Gaussian noise estimate, you can use two good dark frames (time, temperature, and preflash matched to lights). Expect a higher noise estimate. There is more room for error here so I recommend using bias frames to start with.
Thanks,
Mike
PS: Maybe you can dropbox a pair of matching 8300 biases and I will check them out?