Thank you gnomus. The QSI spec
here gives typical high gain 0.16 e-/DN (default), low gain 0.36 e-/DN, and read noise 3 e- RMS in high quality mode. For the script's gain value, specify one of these. For Gaussian noise in DN units, convert read noise in e- to DN by division by gain, eg either 3 / 0.16 = 18.8 DN or 3 / 0.36 = 8.3 DN.
To find a more accurate gain value for your camera, open a frame, choose File > FITS Header, choose the frame's view, and scroll through the list to find the EGAIN keyword. Use this value for the script's gain, and again divide 3 by this value to get Gaussian noise in DN.
To find a more accurate Gaussian noise value, open two good bias frames and run the script DarkBiasNoiseEstimator. Select the two views and click Estimate. Use the temporal noise value which is in DN.
As a sanity check, both EGAIN and the temporal noise value from DarkBiasNoiseEstimator should be roughly equal to the spec values (noise after division).
Best,
Mike
PS: Some vendor drivers do not include the EGAIN keyword, or the value may be generic and not specific for the camera. In this case, to find a more accurate gain value for your camera, open two well-exposed uncalibrated flat frames and one good bias (or flat-dark) frame and run the script FlatSNREstimator. Select the three views appropriately, select 20% as the foreground quantile, and click Estimate. Use the gain value which is in e-/DN units.