that kind of "rain" pattern usually indicates 2 things:
1) there is differential flexure between the guider and the imager. a really long subexposure would show star trails in that direction even though the guider thinks everything is working great
2) the darks don't exactly match the lights and hot pixels were undercorrected.
the foolproof cure to #1 is to use an OAG, but this is really difficult with a DSLR. they do exist, but perhaps only for canon bayonets. still, a super pain in the butt. alternate cures are to stiffen up everything in the imaging and guiding train as much as possible, and make sure neither camera is sagging at all.
#2 is kind of a problem in general with DSLRs. there's no temperature control, so it's hard to get darks that match your lights exactly. these marching pixels are probably hot pixels that were not fully calibrated away.
there are a couple threads here where people were discussing alternate techniques for integrating DSLR darks and how to apply them in ImageCalibration. i can't remember the upshot now, but the solutions were somewhat unorthodox (something like turning on normalization for the darks during dark integration, but don't quote me on that
![smile :)](http://pixinsight.com/forum/Smileys/default/smile.gif)
).
in the short term you might experiment with turning dark optimization on and off, or perhaps using cosmeticCorrection to try to get rid of any residual hot pixels in the calibrated results. or, its possible that by tweaking your ImageIntegration rejection parameters during the light integration that you can get these hot pixels rejected.
rob
rob