Hello,
I have made a quick test, I think you'calibrate the dark twice (once explicitly, and once in the II of the lights).. I get better result by selecting the master bias, dark and flat, but using NO calibrate option. If you look at the histograms of the bias and dark, you will see that the dark is already much darker than the bias, so it was calibrated.
So was the master flat as you mentioned.
The optimize makes no visible difference in this case (factor around 1.01).
The result is not perfect, but well within the reach of a DBE I think, if you increase the tolerance and make sure to stay away of the galaxie with the sample.
-- bitli