Bias frames should have the same ISO of your lights and darks. Since your flats are at a different ISO, take also bias for those flats. 50 may not be enough for this camera, so go for 100.
If you are using an ISO for the lights, be consistant and take the flats, darks and bias at the same value, of things are going to be screwed. Also, double check that you are using bias frames as bias, darks as darks, etc.
Is not the camera that is rotated, it is field rotation due to polar misaligment. So, for normal DSO pictures, you'll have a small amount of rotation between frames. Did you use the first or last frame as the reference?
Anyway, these vertical lines are very wierd, and should not be that consistant unless there is something very wrong with the bias frames. Could you upload your master calibration frames and one uncalibrated light?
(I assume that you always shooted in raw format... if not, there is your answer).