Thanks for the response.
I have been using Image Calibration. I'm not now at the relevant computer, but the essence of the settings is in my text: for the Master Bias, the Master Dark and the Master Flat I either selected the calibrate check-box or not. If not I get the "bad" result, if yes, I get the "good" result.
There is also a checkbox and text entry window (defaulting to 1024) for Optimise in the Master Dark pane. If I leave that selected (default) then in this case the dark does not remove hot pixels. If I deselect it, the hot pixels are mostly removed.
The darks have the same duration and temperature as the lights (-20C and 600s in this case).
The Master frames were made using Image Integration, from more than 50 sub-frames (I forgot the exact numbers).
Thanks,
Richard