Georg suggested that any process for correcting the vertical bars should be applied before the flats. I never thought about it, but he is correct. Just like darks, the band correction is an addition or subtraction process and should be applied before flats. So the question is, is it worth the inconvenience to do it correctly? Often times, with pretty pictures, it does not make a difference. Of course, with science, math processes must be applied in the correct order.
After some testing, I got inconclusive results. In my files, I found data for four sets of images that included flats, darks and the raw sub exposures. These images were also taken with a chip temperature of -25°.
I ran CCD Band-Aid twice for each set of sub exposures, about 30 for each image. This was after applying darks, but before flats and after applying darks and flats. In each case, CCD Band-Aid calculated a more aggressive correction when used before the flats.
Since the correction is added to the dark bands, the signal in all images increased. When checking the background areas, two of the images had an increased signal to noise ratio, and for the other two, it was reduced. The difference was small, only about 3 to 5%. In the case where the S/N decreases, CCDBand-Aid's result may have been an overcorrection. These results were determined with mean combines of dithered sub exposures.
Most importantly, the difference in the images was not detectable visually. In all cases, CCDBand-Aid effectively removed the bars.
Regards,
Bob