if your masters have not been bias subtracted, then the bias signal is present there. in that case you need to tick "calibrate" under the master dark and master flat areas of IC while calibrating your lights.
if the dark subs were bias subtracted before integrating, then you leave "calibrate" unchecked while calibrating your lights. similarly if the flat subs were bias subtracted or had flat darks subtracted, then leave "calibrate" unchecked while calibrating lights.
double bias subtraction would only happen if you pre-subtracted your masters and also ticked 'calibrate'.
i always precalibrate my dark and flat subs, then load a master bias, so i never check "calibrate"
rob