Knro,
I have an idea since it was a problem I had when I first started out trying to do flat frames. One of the critical factors is that everything has to stay exactly the same as when you took your lights. If anything moves, even a little, the flats just won't work and in fact will make things much worse becasue now instead of subtracting the dust circles, you are simply adding new ones in different places. You need to make sure that you are taking your flats right after you take your lights and before any part of your image train changes. That means not refocusing, not removing the CCD from the telescope, not moving the telescope from its mount, etc., etc. Also, you may need to consider a different method for shooting flats.
I currently use a flat panel from Alnitak for my CDK12.5, but they also have smaller sizes and I used one of their smaller versions when I was shooting with my TeleVue 101. Before that I built a light box using the instructions in Richard Berry's Handbook of Astronomical Imaging. Those methods will give you a cleaner light and allow you to control the timing of the flat by dimming or brightening the light. If you decide to stick with using your computer screen, you might want to consider putting a difuser on the front of the OTA. Something as simple as stretching a tee shirt over the front will help.
As to shooting the flats, I used to shoot with an 8300 as well and an ADU count in the 20,000 to 30,000 range is where you want to be. You want a nice high count (so a floor of 20,000) but not too high so you don't bump into the non-linear count when the anti-blooming chip kicks in (thus the ceiling of 30,000). I try to stay around 25,000 but anything in the range with work fine. Also, try to shoot your flats for a little longer. Everything I have read says your flats should be between 2 and 10 seconds. I shoot 3 seconds for RGBs though narrowband filters can take up to 20 seconds, even when I set my light panel to its highest setting.
I hope at least some of that helps,
Best of luck,
Jim
As to the timing of the flats, I was using