Hi Julien,
The problem is with your bias and light frames. They are 16-bit signed integer images storing physical data. By default, PixInsight interprets signed integer images as representing logical data in the range from black=-32768 to white=+32767. This is done to ensure usage of the whole 16-bit numeric range to store pixel values.
These files (your bias and light frames), however, are only using the positive half of the 16-bit range, from black=0 to white=+32767, to store raw CCD data. The negative half from -1 to -32768 is simply not used, which means that the effective bit depth of these images is 15 bits. In our opinion, this is a conceptual error because raw CCD data are unsigned 16-bit integers in the range from 0 to 65535 (and in the case of a light frame, you are probably losing data by storing them with just 15 bits).
To load these files correctly in PixInsight, write the following in the input hints field of ImageIntegration (Format Hints section) when you create your master bias frame:
signed-is-physical
The same applies to the ImageCalibration tool, when you calibrate your light frames. With the above format hint, the FITS support module will know how to interpret these signed integer images.
I strongly recommend you change the necessary settings in your image acquisition software in order to generate exclusively unsigned 16-bit integer images to store your raw data. This is the only correct way to store them.