Carlos, your Debayered Master Calibration levels don’t look like my levels.
My Canon T1i creates a Debayered .fit Bias of 0.0156xx for the R-G-B channels, you should never have 0.0 level in your Bias subs. I would take the time to take one Bias frame at iso100-1/4000 and one Dark frame at iso400-120sec and one Flat frame at iso200 and a speed that will give you a Debayered .fit level of around 0.03 ADU.
Convert these raw files, one bias, one dark and one flat to .fit files and use them as your Master Calibration files.
Now Calibrate the raw dark frame and flat frame and one of your raw light subs and Debayer these new _c.fit files; what levels do you have in these calibrated files?
I’ve attached an example of a similar test, note the small difference between my normal Calibration (40 bias and 10 darks) and the results of Calibration when one frame is used for a Master Bias and a Master Dark.
My Debayered flat .fit level is, R 0.0254xx, G 0,0315xx , B 0.0275xx before Calibration --
after Calibration , flat_c.fit R 0.0095xx G 0.0155xx , B 0.0113xx.
The basic difference in the Debayered flat level is subtracting the 0.0156xx bias level from the raw flat.
Cleon