Tasos and Juan,
Thanks so much for your help. I feel a bit silly that I was unable to find these threads myself.
While I understand what the issue is, I have one more question about the flats I take in AA5. As I understand it, AA5 will use 16 bit signed to save bias frames and 16 bit unsigned to save longer frames (darks, lights etc..). My flat frames are about 6 seconds in length and in AA5 and Nebulosity show a mean of ~25K ADU.
Making the assumptions outlined above (that this image is also 16 bit signed), I checked the box to enable "Signed integer images store physical data". Opening this frame with this option on or off yields an image with a mean of ~63K. I am almost certain this is user error, but I am not exactly sure what I am doing wrong. Any thoughts on this?
I should also note that, checking this box and opening a bias frame works much better. One slight difference is that the mean, as indicated by Nebulosity and AA5 is 914 ADU. When PI opens it, the image shows a mean on ~1800 ADU. This is probably not significant enough to affect a light image during calibration, but I though I would point it out...
To test this, I opened an AA5 bias frame without the "Signed integer images store physical data" checked and verified the mean ADU was around 33k. Using the RGB/K: $T-0.5, the image is manipulated such that the mean ADU is the expected 900. Nor sure why checking the "Signed integer images store physical data" doubled the mean ADU in this case.
Regards,
Ken