Hi Ioannis,
The FIT used can be downloaded from http://www.roryt.gr/astronomy/bias.zip
This FITS file stores a 32-bit floating point image. *This* is the problem. Due to the lack of standardization of the FITS format (others call it 'flexible') that we have commented countless times, PixInsight has no way to know the numeric range to which the stored data has been referred (that is, where is black and where is white). PixInsight always generates floating point images in the [0,1] range, but other applications apply their own (usually undocumented) rules. In practice, this prevents sharing floating point data between applications.
The only reasonable solution is generating raw data as it *should* be generated: unsigned 16-bit integers. This is the native format of CCD cameras and there are no problems at all with it.
Having said that, it seems you can load this image correctly if you do the following:
- Open the Format Explorer window.
- Double click on the FITS format item, left column.
- On the FITS Format Preferences window: (1) click the Reset button, (2) enter 65535 as the Upper Range value of Default Floating Point Input Range, and (3) click OK.
Now you can load this bias frame. Apparently this FITS file stores 16-bit integers as 32-bit floating point numbers. As you surely know this does not mean that you have more precision; it just means that you are wasting twice the required disk and memory space.
Finally, don't forget to reset FITS Format Preferences when you are done, or any floating point FITS file generated with PixInsight won't load correctly.