Hi,
I was just going to reply the other thread when I saw your message. :-P
Overscan correction works in PI by subtracting the pedestal defined by the median value of the overscan of each frame. This means that each calibration frame is overscan corrected, as well as each light frame.
You must set first the image area: the CCD area that contains the sensitive photosites. Then you define the overscan area; if your camera creates different overscan areas, define only one of them.
After defining the overscan area, you must define wich area is going to be corrected by that overscan area. In your case, this area will be the same image area of the beggining. As some other cameras may have more than one readout outputs, they have more than one overscan areas; see my article about NGC7331 to see what I mean.
Best regards,
Vicent.