Hi,
I think the issue is that the first set of images were calibrated, aligned and integrated into a single image and the individual sub-frames were deleted. Now "Kmachhi" want to add a further 40 subs to the already created image.
My suggestion is to process the 40 subs into a single image and then something like pixelmath (sub1 + sub2) /2 or something similar.
space is not black
John