I downloaded the unprocessed image and had a quick look at it. There are still quite a number of hot pixels that calibration and stacking didn't catch. Also the stars look odd. Have you examined the rejection images (high rejection especially)?
If you upload the original light frames and calibration files again, I can have a try at them. The zip file from your earlier post isn't available anymore.
The vertical line requires a bit of trickery to remove. Best is, of course, to avoid it alltogether. Large scale Pixel rejection is probably the best tool for this. To remove it from the integrated image, try this with pixelmath
1. Determine at what x-coordinates the line is. I measured it at x = 2639 ... x = 2641
2. Enter the following formula in PixelMath and execute
iif(x()==2639,pixel($T, x()-1, y()), $T)
3. Next, enter the following formula in PixelMath and execute
iif(x()==2641,pixel($T, x()+1, y()), $T)
4. Then for the middle line
iif(x()==2640,(pixel($T, x()+1, y())+pixel($T, x()-1, y()))/2, $T)
This replaces the outer columns of the line that is 3 pixel columns wide with the neighbouring columns, and the middle pixel column with the average of its neighbours.
However, this leaves a nasty streak. Fill it in with noise through a mask.
Create a mask using PixelMath. Enter the formula
iif(x()>=2639 && x()<=2641,1, 0)
This time set the colourspace in PixelMath to grayscale and create a new image called LineMask.
Apply this mask to the image and use NoiseGeneration to fill in the columns with some noise. Experiment with the amount. I got ok results with Poisson noise and an amplitude of 0.03.