1) Short-term measure
I assume that you did not change the 'RAW format preferences' until now, and that you don't use a rotator for compensating the orientation after M.F. If the issue that you observed is caused by the flip information that the camera stores in the images, you will see it when blinking the uncalibrated light frames in PI: are the images captured after M.F. upside down (indicating no rotation in PI) or in the same orientation as the images captured before M.F. (indicating a rotation by 180° in PI)?
The settings for the RAW module in PixInsight will have an effect only when processing RAW image files (CR2). Since you are using CR2 files, please check the current settings of the RAW module in PixInsight:
Click in the menu 'VIEW/Explorer Windows/Format Explorer'. The Format Explorer then shows a list of format categories. Double click on 'RAW' opens the 'RAW Format Preferences'. Take a screen section of the 'RAW Format Preferences' and show it here.
With this information, the most reasonable way to process all of your present project's light frames to yield a low-noise integration should be obvious.
2) Long-term measure
For future projects it would be worthwhile to revisit which file format is favorable: CR2 or FITS. SGP allows you to save either format, CR2 or FITS (or to save both). I consider using FITS as superior since there is valuable information in the FITS header. Only one important information is missing in the FITS files: the debayer pattern (for the 60D: 'RGGB'). So using the FITS format, you will have to input the debayer pattern 'RGGB' manually when debayering.
If the rotation that you observed is caused by the flip information that the camera stores in the images, this would only happen with the CR2 format AND the wrong setting of the 'No image flip' option in 'RAW Format Preferences', but it would not happen with the FITS format.
It is right to use only one file format. As Rob wrote, mixing images in CR2 and FITS format will not work. So if you decide to change the file format to FITS, you will have to capture new calibration files in the same format.
Regardless of which file format you decide to use in future, in my view it is not a good idea to capture light frames and calibration frames with different software - this could give rise to incorrect image calibration. So yes, I suggest you to use only one acquisition software for all frames in future.
Bernd