As I wrote before, you seem to have neglected the Debayer step before aligning the images.
Don't use Superbias. For the calibration of flat frames use either a MasterBias or a MasterFlat-Dark (made from dark frames captured with the same gain, exposure time and temperature as the flat frames).
MasterDark, MasterBias or MasterFlat-Dark can be reused for some months. MasterFlat can be reused as well, provided that
- the camera was not rotated relative to the scope and
- there is no new contamination with dust particles.
Thus steps 1) to 3) will not be necessary for the next project.
Up to step 4), the results are monochrome CFA images, from step 5) the results are RGB (color) images.
1) Preparation of the MasterDark
Integrate the dark frames (captured at same gain, exposure time and temperature as the light frames)
ImageIntegration, Settings:
Section 'Image Integration'
Combination: Average
Normalization: No normalization
Weights: Don't care (all weights = 1)
Section 'Pixel rejection (1)'
Rejection Algorithm: Winsorized Sigma Clipping
Normalization: No normalization
Save the MasterDark in xisf format, 32-bit IEEE 754 floating point.
2) Preparation of the MasterBias or MasterFlat-Dark
Integrate the bias frames (captured at same gain and temperature as the light frames) or the flat-dark frames (captured with the same gain, exposure time and temperature as the flat frames)
ImageIntegration, Settings: see preparation of the MasterDark
Save the MasterBias or MasterFlat-Dark in xisf format, 32-bit IEEE 754 floating point.
3) Preparation of the MasterFlat
3a) Calibration of the flat frames (captured at same gain and temperature as the light frames) with the MasterBias
ImageCalibration, Settings:
Check section 'Master Bias'; select the MasterBias or MasterFlat-Dark; uncheck 'Calibrate'
Uncheck section 'Master Dark'
Uncheck section 'MasterFlat'
3b) Integration of the calibrated flat frames
ImageIntegration, Settings
Section 'Image Integration'
Combination: Average
Normalization: Multiplicative
Weights: Don't care (all weights = 1)
Section 'Pixel rejection (1)'
Rejection Algorithm: Winsorized Sigma Clipping
Normalization: Equalize fluxes
Save the MasterFlat in xisf format, 32-bit IEEE 754 floating point.
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4) Calibration of the light frames
ImageCalibration, Settings:
Uncheck section 'Master Bias'
Check section 'Master Dark'; select the MasterDark; uncheck both 'Calibrate' and 'Optimize'
Check section 'MasterFlat'; select the MasterFlat; uncheck 'Calibrate'
5) Debayer the calibrated light frames
Debayer, Settings:
Bayer/mosaic pattern: RGGB
Demosaicing method: VNG
6) StarAlignment
Align the calibrated and debayered light frames
Select the reference image (image with the best star attributes, i.e. low Eccentricity and low FWHM)
7) Integration of the calibrated, debayered and aligned light frames
ImageIntegration, Settings:
Section 'Image Integration'
Combination: Average
Normalization: Additive with scaling
Weights: Noise evaluation
Section 'Pixel rejection (1)'
Rejection Algorithm: Winsorized Sigma Clipping
Normalization: Scale + zero offset
Save the integration in xisf format, 32-bit IEEE 754 floating point.
Bernd