Hi Adam:
Attached is a screenshot of the two master darks.
The .xisf is, of course, produced on one of the machines running the latest version of PI.
The .fits master was produced on the older machine that still gives me good calibrated Ha subs.
OK. So I think we can conclude that the calibration process itself (that is adding, subtracting..etc) is not the issue on the old or new machine.
The issue deals with the reason the darks are incompatible with the data.
The fact that you have two distinctly looking darks is a pretty big issue (which is why I gently tried to pull you in this direction.)
My guess is that Steve is correct. These are likely identical darks if you did create them from the same files. I am assuming this is a master dark. If it is a single dark- then certainly there is a geometric transform (rotation, mirror...) in play.
Unfortunately it is degenerate to determine whether the axis/origin is being affected on either the darks or the actual Ha data. The confusing part is that your calibration problem on the new machine should happen for all data... not just the Ha.
Conclusions:
1. Your Darks on the two machines do not match. This is very odd and the explanation is the answer to your problem. It is as if there is a mirroring or rotation of 180 degrees. I can't tell with this small images based on the hot pixels which it is.
2. I have seen problems like this when people use a GEM for acquiring data. At some point data is rotated due to a flip- and this breaks the correspondence with the dark/bias/flat.
3. I guess I should explicitly say that biases and darks have fixed patterns in terms of that gradient (that is what is giving away the problem). I will go a step further and say that the XISF dark is likely in the wrong orientation. Gradients for all STLs I have used are darker at the top and brighter towards the bottom. Heh... I guess it pays that I have that much experience eh?
You should be able to produce a master dark in XISF that is in the correct orientation and will calibrate your Ha. If this same dark DOES NOT calibrate your other LRGB data..it means *that* data is rotated/mirrored..whatever.
Rob: You didn't consider it..but I did. lol this is why I wanted to see a comparison..I did not want to assume (correctly) that the darks were the same.