PI Processing - What Are These Dark Spots?

[jwaters125]

See the picture below.  What's causing these dark pixels?  Are my Sigma High / Low values set wrong?  These dark pixels are all over the place.

Ha Modified Canon 6D
Canon 200mm f/2.8L II lens
BackyardEOS

52 Lights (80 Sec), 60 Darks (80 Sec), 60 BIAS and 40 Flats

I did the Darks in two batches.  The first 20 Darks I did right after I did my Lights.  I added the second batch of 40 Darks today and reprocessed.  I used my refrigerator for the second set of Darks.  I thought the two sets of Darks were within 3 to 5F degrees max.  It turns out they were 8 to 10F degrees apart.

Steps so far:
- BatchPreprocessing
- ImageIntegration
- DynamicCrop
- DynamicBackgroundExtraction
- MultiscaleMedianTransform

More info here
https://www.cloudynights.com/topic/651534-dslr-pixinsight-processing-what-are-these-dark-spots/#entry9164991

Could the Dark temperature differences cause this problem?

[pfile]

did you have dark optimization turned on during calibration?

that can sometimes cause hot pixels to be overcorrected and leave you with these black pixels.

rob

[jwaters125]

Yes