Hi, dark frames won't remove amp glow if you optimize them. I don't think hes referring to that. He is is quite specific in saying that there bias changes every time the exposure or gain is changed.
OK, and did you check what he suggests? Are the results that bad?
I don't own a ASI224 but a ASI294. However, with the ASI294 offset is applied automatically, and there is no problem at all with changing bias.
With the above table would my Electrons Per Data be 0.80 because I used 150 gain? What do I do if I've used tow sets of data at different gains?
Roughly, yes. According to the graph on the ZWO website it is approximately 0.9 electrons/ADU at a gain of 150.
Two sets of data at different gain of course would have to be calibrated separately, with Masters that are generated from calibration frames with suitable gain/offset (same settings as at light frame acquisition).
In practice you will not change gain continually, but you will have to generate Master calibration frames for some exposure times that are used more often.
Bernd