Thanks for all the information and insights.
However, I'm still wondering about Warren Keller's suggestion that a Master Bias + a Defect Map (as AstroArt defines the term) can substitute for Dark frame reduction. In his tutorials on IP4AP, alongside presenting a lot of PI methodology, Warren Keller discusses AstroArt 5 preprocessing (Soup to Nuts #6 and #11)and says the following:
"Before putting time into making many Dark frames, bear in mind that most artifacts, except for hot pixels, are contained in the bias [frames]. Consider Master Bias subtraction and the averaged subtraction of a defect map in lieu of Darks."
This makes a lot of sense to me and it is what prompts my question in a user forum where I see little mention of this methodology. BTW, AA also offers capability to deal with column and row defects.
I'm still wondering if any of the practitioners on this forum have tried this methodology and what recommendations you may have.
I think many (most) people here will have tried all combinations of the above, plus several others, at one time or another. Personally, given the time taken to acquire good image data, I wouldn't even think about dropping dark frames from my processing just to speed things up a little...
Right now I'm going back over some old data (right now some stuff from 2014) with the archived calibration files, and fine tuning the calibration process just to get a slightly cleaner starting point. I have bias, dark and flat frames, and then I run a cosmetic correction process, taking into account the column defects that I can see in contemporaneous dark frames.
Generating a decent bias and dark frame set really doesn't take much time. I refresh my bias and dark frames about every 3-6 months (the camera chip ages noticeably on that sort of timescale). It takes a single overnight run to capture a new set.
For your setup (very nice!) the QSI camera has a thermoelectric cooler with good regulation (I have the older QSI583 myself, which has the same KAF-8300 image sensor). I would strongly recommend finding a temperature that you can comfortably hit under all reasonable conditions and standardize on that. That way you won't have to worry about creating a calibration library with multiple different temperatures. I use -10C. Chris above recommends -20C. Depending on your local temperature conditions, you may struggle to hit -20C with the cooler in that camera (just based on my own experience with the 583). You should experiment a little with this; run the camera indoors at room temperature, and see if you can get to -15C or -20C without the cooler running at 100% power.
You can always start with a standard set point of -10, and then go colder later on. Realistically, there are a lot of things you are going to have to get ironed out before dark current becomes your biggest problem...
Most importantly: start taking images as soon as you can. You're going to find there's a lot of bugs in your setup that you have to work out, and a lot of procedures that you have to master, one at a time. The sooner you get outside and start switching things on and finding what works the better.
Good luck!