Note, while I'm at it... I went ahead and stacked 402 total bias RAWs at ISO400 from my 450D (and this time set the high/low sigma to 10.0 each while using BPP to script a stack).
To me (as expected), I saw barely any difference between the 402 count stack and the 202 count stack in the resulting master bias. That's expected since I've read that not much past 50 offers really noticeable improvement. That being said, is it possible to have too many? Other than you'll just mostly end up wasting integration time.
THEN, just for fun, I created a Superbias using this new master-bias created by the 402 count of bias RAWs (1/4000 exposure at ISO400). I did it twice, once with the Multiscale Layers defaulted to 7, and again with the max value of 10 (I haven't messed with any of the other parameters of superbias). The only real difference I see visually with the Multiscale 10 version of the super-bias from the 402 count stack is that it has less "haze" along the top.
And not that it matters too much, but another observation is that the superbias files are less than half the filesize as the regular masters.
The additional master-bias combinations are available at the share:
https://app.box.com/s/hjs0mt9i7b90xvzaa5jvAgain, the 402 count stacked bias (with 24-bit AutoSTF) didn't look much different than the 202 count stack. But attached here is a side-by-side comparison of the Multiscale 10 (right side) versus 7 (left side). I would suspect a superbias of the 202 stack wouldn't look much different either.
Question is: any point in using a superbias of such a large number of stacked biases already?