I have attached a screenshot of 5 calibration frames taken with the same DSLR under the same conditions, except control of shutter speed. This may be of interest - the results are interesting.
Centre image for comparison - 100iso bias, 3 frames, 'zero' shutter speed. Arduino micro-controller scheduling shutter speed - on/off no delay
RGB ~480
Top left - 800iso bias, 3 frames, camera max shutter speed 1/8000 sec
RGB ~86
Bottom left - 800iso dark, 3 frames, 210 secs.
RGB ~3
Top right - 800iso bias, 'zero' shutter speed 3 frames - Arduino...
RGB ~278
Bottom right - 800iso dark 210secs 3 frames
RGB ~3
The darks are virtually identical regardless of the bias used.
Note: I'm not sure of the actual shutter speed using the Arduino - bypassing the camera electronics.