Hi Rich,
Let's look at some of the statements in your last post :
my Master Dark and MasterBias are debayered stretched RGBs. Therefore they are not usable
for proper calibration . . .
I would have to agree - when you are trying to calibrate images, you want to be working with 'un-processed' data, and certainly not with data that has been processed in-camera using algorithms that are not made available to the user.
. . . noisy camera with thousand of warm/hot pixels which need to be removed
There is nothing particularly 'noisy' about your specific DSLR camera -
all cameras (including high-end, multi-$10K imagers) are 'noisy'. The difference comes with how the noise is treated after an exposure is made. In 'our world' of astro-imaging we learn how to categorise the noise and then, with that information, we learn to take the necessary steps to counteract the effects of the noise (note: I didn't say "eliminate the noise" - in fact, that isn't even our ultimate goal). But, your DSLR is not targetted towards astro-imaging, it is a 'simple device' (no matter how much it costs!!) that is targetted at a wide range of users and scenes - including the ability to take long exposures in low-light conditions. But, that ability does not even begin to get close to the long exposures and low-light conditins of an astro-image.
Your DSLR will try to do the best job that it can, but it will ultimately fail - leaving you with an image that still contains noise. The problem is that, now, it is no longer possible to categorise that noise and so treatment cannot proceed in the usual manner (Darks, Biases, Flats, etc.) - if at all.
Biases are to be taken at the lowest ISO . . . / . . . match the ISO of your lights
How to know which is correct? Simple - you have to run an experiment, and determine the answer emprically. Take two groups of 30 Biases, oerform the simplest ImageIntegration on each group, and then look at the resultant Statistical Information for each MasterBias. Of course, if you can figure out a way of getting the DSLR to simply give you the RAW image, that helps a lot!
You can also think of things this way - on many (most? / all?) astroimaging cameras, the user has the facility to be able to set Gain and Offset (personally, I wouldn't buy an imager that didn't give me access to these two crucial parameters). There is a well-established routine that allows a user to determine the optimum Gain and optimum Offset for their imager and, once correctly set, these should remain reasonably stable throughout the life of the imager (aklthough easy enough to check, perhaps tweak, at any time in the future). Once these two parameters have been set, all future images are acquired using these parameters - including all calibration frames.
Now, in simplistic terms, your ISO setting is the equivalent of the Gain parameter, but you have no equivalent (IMHO) of the Offset parameter (which, more or less, determines the base ADU value for a Bias frame). So, in trying to answer your question, I might suggest that you select a fixed ISO (not necessarily the 'highest' figure that your camera can achieve - you can still use the algorithm that CCD imagers rely on) and use that for all of your imaging work, including calibration frame acquisition.
In my earlier post, I said :
at the lowest ISO rating the camera will offer
In my defence, that statement was to try and establish what the DSLR actually used as its 'base level' (I leave you to determine just how much snake-oil I am trying to sell with this statement
)
. . . the noise reduction is done internally, so there are not 2 files . . .
That is also entirely plausible - after all PixInsight itself has a multitude of 'noise-removal' processes in its armoury. But, we come back to the same thing - if you only take a
single long-exposure in low-light conditions, then this one-off image might well be improved by letting the DSLR work its magic. However, you then gain nothing by taking further, similar, images with the intention of stacking these using ImageIntegration. Well, that's not strictly true - you do gain something - more noise
Why? Well it all comes down to understanding how the (tried and trusted) calibration process works - at a statistical level. There are plenty of good sources of information on that subject - and not too many snake-oil dealers. In summary though, because you have allowed the DSLR to 'change' the source image, you no longer have the raw data that is required to be able to reliably use the normal calibration process.
Again, I hope this helps, and I am happy to stand corrected on any or all of the comments or explanations that I have made.