Geoff,
Thanks for your reply, but I must admit to being a bit confused.
With 1x1 binning, pixel to pixel variation can be corrected. However, it is in the nature of a 2x2 or 3x3 binned image, that pixel to pixel variations are no longer possible, because of the binning. We have new "super pixels" made up from combinations of actual pixels.
When an image is binned in the camera, the photons collected from each super pixel are collected. When resampling a 1x1 binned image to 2x2, I had understood that more or less the same thing happens - i.e. the values of adjoining groups of pixels are combined in the software to make "super pixels". Is that an incorrect understanding?
Are you saying that we lose the possibility to correct pixel to pixel variations by using a binned flat? That is obviously true, but what is the alternative when calibrating binned lights? PixInsight will not accept a flat where the binning differs from the light to be calibrated, at least when using the batch processing script.
I'm sure that my technical understanding is incomplete or just wrong, but would appreciate some further explanation.
All the best,
Stephen