ISO setting is just a gain, so if you calibrate an iso800 sub with an iso1600 master dark (same duration and temperature), you should get a dark optimization factor of around 0.5. This will work only if you do an accurate bias calibration.
Granted that (nonlinear) hot pixels will not be fully calibrated. But if you did some dithering, those can be handled during stacking with outlier rejection.
Ignacio