IanL: what you are effectively doing, by avoiding hot pixels, and now trying to deal with cold pixels, is calibrating your darks with some of their repetitive patterns. Try this: build a master dark with all of your frames. Then use it to calibrate the whole set of darks: dark frame - master dark + small pedestal if needed. Then stack it in increasing subsets as in your exercise, and look at the noise progression. It should decay exactly as expected from theory, at least at the beginning, since as you approach the total number of frames, noise approaches zero (and gets there at the end when the master dark is subtracted from itself).
Ignacio