I do my color calibration after the image has been cropped to ensure rgb borders all around and has had a Dynamic Background Extraction or Background Neutralization process done. As I dither my images, after combining the red, green, and blue images, the border is usually overlapped. Cropping the RGB image ensures that there is RGB data in the entire frame. Now when I do any procedure there shouldn't be any area where one frame is/isn't represented. Now doing either the above and then color calibration I know the entire data set is sampled. Hope this makes sense.