My experience with QSI-683, same sensor as yours.
2400s lights 3nm Ha, 64x 2400s darks taken within one month of lights, all binned 2x2, all at -20ÂșC.
Some "warm" pixels in the lights get warmer than they were in the darks, and so don't calibrate out.
Some "warm" pixels in the lights get cooler than they were in the darks, so the reverse problem.
Some "warm" pixels in the lights were normal in the darks. And so on.
Solution: 1) Don't scale dark master, that only makes the problems worse. 2) Don't use a bias master, just dark-subtract, 3) Dither, good dithering plus good rejection solves the problem completely for me.