Bernd,
Once again, thanks massively for you help. What I think may have happened, (however, see "Finally" below), is that somehow or other I have accidently shifted or not noticed the shift in gain or off-set, or black point it think it is called in SharpCap. So if that is the case I will have to be more observant of settings between imaging runs. (I like to periodically check focus hence the 25 to 30 minutes or so maximum imaging runs.)
I'm just getting into understanding ADUs / unity gain etc - gain 400 is unity I believe for a 183c camera. 500 ADU's seems big difference between the two imaging runs. Maybe I'll request Dr Robin Glover to consider gain and off-set in the FITs headers of SharpCap!
Also, thanks for reminding me of the Statistic process, although I have an engineering based background in statistics, !would not have realised that the answer lay in these numbers. Just to note that these images are 12 bit. But I suspect Ihat the message is the same! So for my own learning I take it that the mean/median of an image should be higher than the mean/median of the dark master?
As for the sensor temperature, yes I have the 183C Pro TEC, however, for the following reasons I chose not to turn it on, (normally I set it to -20C);
1. I have recently moved house and I have not yet installed mains power to my back garden. I was on battery power and chose not to turn the cooling system on to maximise available time.
2. The primary purpose of this imaging session was to test out my new PrimaLuceLab Sesto Senso robotic focuser and to grapple with SharpCap Pro's excellent Smart Histogram and Brain capabilities. I chose M31 core because it was quick and easy to find.
Finally, the Not OK frames were taken before to OK frames? Hope that it is not a fault in the camera. More likely a fault in the operator!
So thanks again - I take my hat off to you depth of knowledge.
Colin