I am having a similar issue with the same sensor on the Altair model (Hypercam 179c). I tried this last night and it did, indeed, remove the glow however it replaced it with absolute black in the same shape as the glow. This is obviously an improvement - however it wasn't what I expected (to be fair, I'm not sure what I WAS expecting!). It is easier to work with than glow but it still leaves a mismatch against the sky background and so still needs cropping. Other software packages seem to do a more precise removal (the latest Sharpcap for example).
I am therefore sure I am not quite doing it right in Pixinsight. In regards to not ticking "optimize dark frames", do I leave it unticked for both calibration of flats AND lights, or just lights? I unticked it for both.
As an alternative, could I use the master darks and flats generated by Sharpcap and just calibrate my lights from those? I know the obvious answer is to try it but, with the batch of 80x60sec subs I am working with, it takes over an hour a time to get to the end of calibrated and stacked lights so if anyone has any tips, I'd appreciate it.
I am really grateful for the OP and answer in this thread as it has moved me on in terms of dealing with my issue. Thanks.